Molecular characterization of mariner-like elements in Bruchus pisorum and Bruchus rufimanus (Coleoptera: Bruchidae)
Mariner-like elements (MLEs) are Class-II transposons that are widely present in diverse organisms and encode a D,D34D transposase motif. MLE sequences from two coleopteran species, Bruchuspisorum and B. rufimanus were obtained using the terminal-inverted repeats (TIRs) of mariner elements belonging...
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Published in | Archives of biological sciences Vol. 69; no. 2; pp. 353 - 360 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
University of Belgrade, University of Novi Sad
2017
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Subjects | |
Online Access | Get full text |
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Summary: | Mariner-like elements (MLEs) are Class-II transposons that are widely present
in diverse organisms and encode a D,D34D transposase motif. MLE sequences
from two coleopteran species, Bruchuspisorum and B. rufimanus were obtained
using the terminal-inverted repeats (TIRs) of mariner elements belonging to
the mauritiana subfamily as primer. The characterized elements were between
1073 and 1302 bp in length and are likely to be inactive, based on the
presence of multiple stop codons and/or frameshifts. A single consensus of
MLE was detected in B. pisorum and was named Bpmar1. This element exhibited
several conserved amino acid blocks as well as the specific D,D(34)D
signature. As for B. rufimanus, two MLE consensuses, designated Brmar1 and
Brmar2, were isolated, both containing deletions overlapping the internal
region of the transposase. Structural and phylogenetic analysis of these
sequences suggested a relatively recent origin of Bpmar1 versus a more
ancient invasion of Brmar1 and Brmar2 in their respective host genomes. Given
that MLEs are potential mediators of insect resistance and have been used as
vectors to transfer genes into host genomes, the MLEs characterized in this
study will have valuable implications for selecting appropriate transposable
elements in transgenesis.
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ISSN: | 0354-4664 1821-4339 |
DOI: | 10.2298/ABS160407111D |