Solid Substrate–Room Temperature Phosphorimetry for the Determination of Trace Terbutaline Sulfate Based on Its Inhibition Oxidation of Rhodamine 6G by Sodium Periodate

• Published in: Journal of fluorescence Vol. 18; no. 2; pp. 573 - 579
• Main Authors: Liu, Jia-Ming, Gao, Fei, Gao, Wen-Yan, Zeng, Li-Qing, Huang, Xiao-Mei, Li, Zhi-Ming, Huang, Xiu-Chai, Lin, Wei-Nv, Wang, Fang-Mei, Nie, Chang-Ling
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.03.2008
• Subjects: Analytical Chemistry; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biomedicine; Biophysics; Biotechnology; Luminescent Measurements - methods; Original Paper; Oxidation-Reduction; Periodic Acid - pharmacology; Rhodamines - chemistry; Spectrometry, Fluorescence; Temperature; Terbutaline - analysis; Inhibition solid substrate–room temperature phosphorimetry; Terbutaline sulfate; Rhodamine 6G
• ISSN: 1053-0509; 1573-4994
• DOI: 10.1007/s10895-007-0301-2

When 1.00 mol l −1 I − is used as ion perturber, rhodamine 6G (Rh 6G) can emit strong and stable room temperature phosphorescence (RTP) on filter paper substrate in KHC 8 H 4 O 4 –HCl buffer solution (pH = 3.50), heated at 70 °C for 10 min. NaIO 4 can oxidize Rh 6G, which makes the RTP signal quench. Terbutaline sulfate (TBS) can inhibit NaIO 4 from oxidizing Rh 6G, which makes the RTP signal of Rh 6G enhance sharply. The content of TBS is linear correlation to Δ I p of the system. Based on the facts above, a new inhibition solid substrate-room temperature phosphorimetry (SS-RTP) for the determination of trace TBS has been established. The linear range of this method is 0.0104–2.08 pg spot −1 (corresponding concentration: 0.026–5.2 ng ml −1 , with a sample volume of 0.4 μl) with a detection limit (L.D.) of 2.6 fg spot −1 (corresponding concentration: 6.5 × 10 −12  g ml −1 ), and the regression equation of working curve is Δ I p = 2.040 + 54.54 m TBS (pg spot −1 ), n  = 6, correlation coefficient is 0.9994. For the samples containing 0.0104 pg spot −1 and 2.08 pg spot −1 TBS, the relative standard deviation (RSD) are 3.8% and 2.3% ( n  = 8), respectively, indicating good precision. This method has been applied to determination of trace TBS in the practical samples with satisfactory results. The reaction mechanism of NaIO 4 oxidizing Rh 6G to inhibit SS-RTP for the determination of trace TBS is also discussed.