Quebrachitol inhibits biofilm formation and virulence production against methicillin-resistant Staphylococcus aureus

• Published in: Microbial pathogenesis Vol. 149; p. 104286
• Main Authors: Vijayakumar, Karuppiah, Bharathidasan, Veeraiyan, Manigandan, Vajravelu, Jeyapragash, Danaraj
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.12.2020
• Subjects: Anti-adherence activity; Antibiofilm activity; MRSA; Quebrachitol; sarA; sarA; Quebrachitol; Anti-adherence activity; Antibiofilm activity; MRSA
• ISSN: 0882-4010; 1096-1208
• DOI: 10.1016/j.micpath.2020.104286

The present study evaluated the quebrachitol (QBC) antibiofilm and antivirulence potential against methicillin-resistant Staphylococcus aureus (MRSA). QBC inhibited MRSA biofilm formation at concentration dependent manner without affecting the bacterial growth. Then, QBC biofilm efficacy was confirmed with light and confocal laser scanning microscopy analysis. QBC treatment significantly inhibited the biofilm formation on stainless steel, titanium and silicone surfaces. Besides, QBC treatment significantly reduced the MRSA virulence productions such as lipase and hemolysis. Moreover, it reduced MRSA survival rate in the presence of hydrogen peroxide. QBC treatment inhibited the MRSA adherence on hydrophobic, hydrophilic, collagen coating and fibrinogen coating surfaces. As well as it significantly reduced the autolysin and bacterial aggregation progress. The real-time PCR analysis revealed the ability of QBC downregulated the virulence genes expression including global regulator sarA, agr and polysaccharide intracellular adhesion (PIA) encode ica. The cumulative results of the present study suggest that QBC as a potential agent to combat against MRSA pathogenesis. •The present study determined the antibiofilm activity of quebrachitol (QBC) against methicillin reisistant Staphylococcus aureus (MRSA).•The present study evaluated the surface independent antibiofilm potential and anti-adherence potential of QBC against MRSA.•To evaluated the QBC antivirulence activity against MRSA.•The geneexpression analysis to evaluate the virulence genes expression upon QBC treatment.•The present study suggest the potential use of QBC as a potential agent to combat against MRSA pathogenesis.