A possible role for the pcnB gene product of Escherichia coli in modulating RNA: RNA interactions

The sequence of the PcnB protein of Escherichia coli, a protein required for copy number maintenance of ColE1-related plasmids, was compared with the PIR sequence database. Strong local similarities to the sequence of the E. coli protein tRNA nucleotidyltransferase were found. Since a substrate of t...

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Bibliographic Details
Published inMolecular & general genetics Vol. 220; no. 2; p. 341
Main Authors Masters, M, March, J B, Oliver, I R, Collins, J F
Format Journal Article
LanguageEnglish
Published Germany 01.01.1990
Subjects
Amino Acid Sequence
Bacterial Proteins - genetics
Bacteriocin Plasmids
DNA Replication
Escherichia coli - genetics
Escherichia coli Proteins
Molecular Sequence Data
Plasmids
Polynucleotide Adenylyltransferase
RNA Nucleotidyltransferases - genetics
RNA, Bacterial - metabolism
Sequence Homology, Nucleic Acid
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Summary:The sequence of the PcnB protein of Escherichia coli, a protein required for copy number maintenance of ColE1-related plasmids, was compared with the PIR sequence database. Strong local similarities to the sequence of the E. coli protein tRNA nucleotidyltransferase were found. Since a substrate of the latter protein, tRNA, structurally resembles the RNAs that control ColE1 copy number we believe that we may have identified a region in PcnB that interacts with these RNAs. Consistent with this idea is our observation that PcnB is required for the replication of R1, a plasmid whose replication is also regulated by a small RNA.
ISSN:0026-8925
DOI:10.1007/BF00260507