On-line polydopamine coating as a new way to functionalize polypropylene fiber sorbent for solid phase extraction

• Published in: Talanta (Oxford) Vol. 219; p. 121189
• Main Authors: Háková, Martina, Chocholouš, Petr, Valachovič, Adam, Erben, Jakub, Chvojka, Jiří, Solich, Petr, Švec, František, Šatínský, Dalibor
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2020
• Subjects: 2D UHPLC; Chlorogenic acid; Fibrous sorbent; On-line coating; On-line solid phase extraction; Sorbent modification; On-line coating; Sorbent modification; Fibrous sorbent; Chlorogenic acid; 2D UHPLC; On-line solid phase extraction
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2020.121189

Effective process, including a cartridge packing polypropylene fiber sorbent modified by following on-line polydopamine coating, for on-line solid phase extraction in 2D UHPLC system has been developed. Hydrophobic surface of mechanically stable polypropylene fibers was hydrophilized using an automated and reproducible in situ coating process to enable good wettability and effective extraction of polar compounds. Polymerization mixture consisting dopamine and TRIS buffer was circulated through the cartridge containing polypropylene fibers using a peristaltic pump to achieve polymerization. This process was optimized in terms of dopamine amount in the polymerization mixture, its flow rate, and polymerization time. Best results were obtained with 25 mL polymerization mixture containing 20 mg dopamine circulated through the cartridge at a flow rate of 2.07 mL min−1 for 60 min. Prepared cartridges were evaluated via measurement of the recovery and reproducibility using chlorogenic acid as a model compound. Overall reproducibility of our multistep process including eight cartridges in 2D UHPLC system, each measured in triplicate, was 3.61% (n = 24). [Display omitted] •Automated on-line polydopamine coating of fibrous sorbent.•Highly reproducible and time effective coating procedure.•Chemical resistant and mechanical stable sorbent for solid phase extraction.•Polydopamine modified meltblown produced polypropylene fibers.•On-line solid phase extraction of chlorogenic acid.