Analysis of Pterins in Urine by HILIC

• Published in: Chromatographia Vol. 71; no. 9-10; pp. 761 - 768
• Main Authors: Shi, Zhi-Guo, Wu, Yu-Bo, Luo, Yan-Bo, Feng, Yu-Qi
• Format: Journal Article
• Language: English
• Published: Wiesbaden Wiesbaden : Vieweg Verlag 2010; Vieweg Verlag; Springer
• Subjects: Analytical Chemistry; Biological and medical sciences; biopterin; Chemistry; Chemistry and Materials Science; Chromatography; Column liquid chromatography; Hydrophilic interaction chromatography; Investigative techniques, diagnostic techniques (general aspects); Isoxanthopterin; Laboratory Medicine; Medical sciences; Metabolic diseases; neopterin; Original; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Pharmacy; Proteomics; urine; Urine; Column liquid chromatography; Biopterin; Neopterin; Hydrophilic interaction chromatography; Isoxanthopterin; Biological fluid; Chemical analysis; HPLC chromatography; Enzymopathy; Clinical biology; Aminoacid disorder; Quantitative analysis; Human; Validation; Nervous system diseases; Fluorescence detector; Hydrophilic Interaction Liquid chromatography; Pteridine derivatives; Elimination; Metabolic diseases; Patient; Normal; Genetic disease; Hyperphenylalaninemia; Reversed phase chromatography; Comparative study
• ISSN: 0009-5893; 1612-1112
• DOI: 10.1365/s10337-010-1567-0

Pterins are a class of compounds excreted in urine. Levels of the pterins are found to be significantly elevated in a variety of diseases. A new method involving hydrophilic interaction chromatography with fluorescence detection has been developed for analysis of neopterin, biopterin, and isoxanthopterin in urine samples. Separation of these pterins on an aminopropyl hydrophilic interaction column was achieved by isocratic elution. The effects of the organic modifier content, ionic strength, and pH of the mobile phase on the hydrophilic behavior of the pterins were studied and the mechanism of their separation was also investigated. Under the optimum chromatographic conditions the linearity (r ≥ 0.9995) and repeatability (relative standard deviation < 4.0%) of the method are good. Compared with reversed-phase high-performance liquid chromatography, the method is simple and convenient. The method was applied to the analysis of pterins in urine samples with satisfactory results.