A Pathogenic Missense Mutation in Kainate Receptors Elevates Dendritic Excitability and Synaptic Integration through Dysregulation of SK Channels

• Published in: The Journal of neuroscience Vol. 43; no. 47; pp. 7913 - 7928
• Main Authors: Nomura, Toshihiro, Taniguchi, Sakiko, Wang, Yi-Zhi, Yeh, Nai-Hsing, Wilen, Anika P, Castillon, Charlotte C M, Foote, Kendall M, Xu, Jian, Armstrong, John N, Savas, Jeffrey N, Swanson, Geoffrey T, Contractor, Anis
• Format: Journal Article
• Language: English
• Published: United States Society for Neuroscience 22.11.2023
• Subjects: Action potential; Afterhyperpolarization; Amino acids; Animals; Brain damage; Calcium conductance; Calcium ions; Channels; Coupling; Dendritic structure; Down-regulation; Excitability; Excitatory postsynaptic potentials; Female; Glutamic acid receptors (ionotropic); Hippocampus; Hippocampus - physiology; Humans; Kainic acid receptors; Kinetics; Long-term potentiation; Male; Mice; Missense mutation; Mutants; Mutation; Mutation, Missense; Neurodevelopmental disorders; Neurons; Neurons - physiology; Phenotypes; Potassium channels (calcium-gated); Potassium conductance; Pyramidal cells; Pyramidal Cells - physiology; Receptors; Receptors, Kainic Acid - genetics; Receptors, Kainic Acid - metabolism; Synapses; kainate receptor; SK channels; hippocampus; dendritic excitability
• ISSN: 0270-6474; 1529-2401
• DOI: 10.1523/JNEUROSCI.1259-23.2023

Numerous rare variants that cause neurodevelopmental disorders (NDDs) occur within genes encoding synaptic proteins, including ionotropic glutamate receptors. However, in many cases, it remains unclear how damaging missense variants affect brain function. We determined the physiological consequences of an NDD causing missense mutation in the kainate receptor (KAR) gene, that results in a single amino acid change p.Ala657Thr in the GluK2 receptor subunit. We engineered this mutation in the mouse gene, yielding a GluK2(A657T) mouse, and studied mice of both sexes to determine how hippocampal neuronal function is disrupted. Synaptic KAR currents in hippocampal CA3 pyramidal neurons from heterozygous A657T mice exhibited slow decay kinetics, consistent with incorporation of the mutant subunit into functional receptors. Unexpectedly, CA3 neurons demonstrated elevated action potential spiking because of downregulation of the small-conductance Ca activated K channel (SK), which mediates the post-spike afterhyperpolarization. The reduction in SK activity resulted in increased CA3 dendritic excitability, increased EPSP-spike coupling, and lowered the threshold for the induction of LTP of the associational-commissural synapses in CA3 neurons. Pharmacological inhibition of SK channels in WT mice increased dendritic excitability and EPSP-spike coupling, mimicking the phenotype in A657T mice and suggesting a causative role for attenuated SK activity in aberrant excitability observed in the mutant mice. These findings demonstrate that a disease-associated missense mutation in leads to altered signaling through neuronal KARs, pleiotropic effects on neuronal and dendritic excitability, and implicate these processes in neuropathology in patients with genetic NDDs. Damaging mutations in genes encoding synaptic proteins have been identified in various neurodevelopmental disorders, but the functional consequences at the cellular and circuit level remain elusive. By generating a novel knock-in mutant mouse, this study examined the role of a pathogenic mutation in the GluK2 kainate receptor (KAR) subunit, a subclass of ionotropic glutamate receptors. Analyses of hippocampal CA3 pyramidal neurons determined elevated action potential firing because of an increase in dendritic excitability. Increased dendritic excitability was attributable to reduced activity of a Ca activated K channel. These results indicate that a pathogenic KAR mutation results in dysregulation of dendritic K channels, which leads to an increase in synaptic integration and backpropagation of action potentials into distal dendrites.