Metabolism of Soyasaponin I by Human Intestinal Microflora and Its Estrogenic and Cytotoxic Effects
Metabolites of Soyasaponin I, a major constituent of soybean, by human intestinal microflora were investigated by LC-MS/MS analysis. We found four peaks, one parental constituent and three metabolites: m/z 941 [M-H]−, m/z 795 [M-rha-H]−, m/z 441 [aglycone-H2O+H]+, and m/z 633 [M-rha-gal-H]−, which w...
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Published in | Biomolecules & therapeutics Vol. 17; no. 4; pp. 430 - 437 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
한국응용약물학회
31.10.2009
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Subjects | |
Online Access | Get full text |
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Summary: | Metabolites of Soyasaponin I, a major constituent of soybean, by human intestinal microflora
were investigated by LC-MS/MS analysis. We found four peaks, one parental constituent and three
metabolites: m/z 941 [M-H]−, m/z 795 [M-rha-H]−, m/z 441 [aglycone-H2O+H]+, and m/z 633 [M-rha-gal-H]−,
which was an unknown metabolite, soyasapogenol B 3-β-D-glucuronide. When soyasaponin I was incubated
with the human fecal microbial fraction from ten individuals for 48 h, soyasaponin I was metabolized to
soyasapogenol B via soyasaponin III and soyasapogenol B 3-β-D-glucuronide or via soyasaponin III alone.
Both soyasaponin I and its metabolite soyasapgenol B exhibited estrogenic activity. Soyasaponin I increased
the proliferation, mRNA expression of c-fos and pS2, in MCF7 cells more potently than soyasapogenol B.
However, soyasapogenol B showed potent cytotoxicity against A549, MCF7, HeLa and HepG2 cells, while
soyasaponin I did not. The cytotoxicity of soyasapogenol B may prevent its estrogenic effect from increasing
dose-dependently. These findings suggest that orally administered soyasaponin I may be metabolized to
soyasapogenol B by intestinal microflora and that soyasapogenol B may express a cytotoxic effect rather than
an estrogenic effect. KCI Citation Count: 16 |
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Bibliography: | G704-000363.2009.17.4.008 |
ISSN: | 1976-9148 2005-4483 |
DOI: | 10.4062/biomolther.2009.17.4.430 |