Evaluating Blood Cell Populations in Xenopus Using Flow Cytometry and Differential Counts by Cytospin

Analyses by flow cytometry and differential counts by cytospin of peripheral blood leukocytes are two reliable and convenient methods used to assess the immune status and immune responses in the amphibian Xenopus. Here, we describe these methods and discuss their challenges and limitations.

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1865; p. 265
Main Authors Robert, Jacques, Edholm, Eva-Stina, De Jesus Andino, Francisco
Format Journal Article
LanguageEnglish
Published United States 01.01.2018
Subjects
Animals
Blood Cell Count - methods
Blood Cells - cytology
Cell Separation
Centrifugation
Flow Cytometry - methods
Xenopus laevis - metabolism
Aquatic vertebrates
Developmental immunology
Comparative immunology
Amphibian
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Summary:Analyses by flow cytometry and differential counts by cytospin of peripheral blood leukocytes are two reliable and convenient methods used to assess the immune status and immune responses in the amphibian Xenopus. Here, we describe these methods and discuss their challenges and limitations.
ISSN:1940-6029
DOI:10.1007/978-1-4939-8784-9_19