Casting Zinc Oxide Nanoparticles Using Fagonia Blend Microbial Arrest

• Published in: Applied biochemistry and biotechnology Vol. 195; no. 1; pp. 264 - 282
• Main Authors: Hussain, Riaz, Zafar, Ayesha, Hasan, Murtaza, Tariq, Tuba, Saif, Muhamamd Saqib, Waqas, Muhammad, Tariq, Fatima, Anum, Muniba, Anjum, Syed Ishtia, Shu, Xugang
• Format: Journal Article
• Language: English
• Published: New York Springer US 2023; Springer Nature B.V
• Subjects: Amino acids; Anti-Bacterial Agents - chemistry; Antiinfectives and antibacterials; Antimicrobial agents; Bacteria; Bacteria - metabolism; Bioactive compounds; Biochemistry; Biological activity; Biotechnology; Carbohydrates; Chemistry; Chemistry and Materials Science; Coliforms; E coli; Fagonia; Flavonoids; Gram-Negative Bacteria; Gram-Positive Bacteria; Metal Nanoparticles - chemistry; Microbial Sensitivity Tests; Microorganisms; Nanoparticles; Nanoparticles - chemistry; Original Article; Phenols; Plant extracts; Plant Extracts - chemistry; Production methods; Proteins; Saponins; Spectroscopy, Fourier Transform Infrared; Stabilizers (agents); Steroid hormones; Wurtzite; Zinc oxide; Zinc Oxide - chemistry; Zinc oxides; Plant extract; Antimicrobial activity; Green synthesis; Zinc NPs; Bioreducing agent
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-022-04152-8

Physical and chemical methods for production of nanoparticles (NPs) are not only harmful for environment but also toxic for living organism. The present study attempts to synthesize ZnO NPs using the natural plant extract of Fagonia cretica . The phytochemical screening of F. cretica water extract was performed to check the presence of biologically active compounds like alkaloids, tannins, carbohydrates, proteins, phenols, saponins, flavonoids, and steroids. Well-prepared ZnO NPs given sharp absorption peak at 362 were confirmed by UV–visible. XRD analysis showed the ZnO NPs having wurtzite hexagonal structure with crystalline form. TEM analysis endorses flower-shaped ZnO nanoparticles ~ 100–1000 nm. FTIR spectrum suggested the involvement of phenolic groups and amino acids and amide linkages in protein performs as the stabilizing agent in the synthesis of ZnO NPs. The ZnO NPs showed strong antibacterial behavior against two bacterial strains Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria Escherichia coli . In addition, ZnO NPs exhibited strong antioxidant activity of 79%:85.6%:89.9% at 5 μg/mL:10 μg/mL:5 μg/mL concentration of ZnO NPs respectively. This work indicates that Fagonia is considered to be appropriate and promising candidate for extending the innovative applications in the field of medicine and industry and also helpful and useful to the scientific communities.