Resistance of mitochondrial DNA to cadmium and Aflatoxin B1 damage-induced germline mutation accumulation in C. elegans

• Published in: Nucleic acids research Vol. 50; no. 15; pp. 8626 - 8642
• Main Authors: Leuthner, Tess C, Benzing, Laura, Kohrn, Brendan F, Bergemann, Christina M, Hipp, Michael J, Hershberger, Kathleen A, Mello, Danielle F, Sokolskyi, Tymofii, Stevenson, Kevin, Merutka, Ilaria R, Seay, Sarah A, Gregory, Simon G, Kennedy, Scott R, Meyer, Joel N
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 26.08.2022
• Subjects: Aflatoxin B1 - toxicity; Animals; Cadmium - toxicity; Caenorhabditis elegans - genetics; DNA, Mitochondrial - genetics; Genome Integrity, Repair and; Germ Cells; Mitochondria - genetics; Mutation; Mutation Accumulation
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/gkac666

Abstract Mitochondrial DNA (mtDNA) is prone to mutation in aging and over evolutionary time, yet the processes that regulate the accumulation of de novo mtDNA mutations and modulate mtDNA heteroplasmy are not fully elucidated. Mitochondria lack certain DNA repair processes, which could contribute to polymerase error-induced mutations and increase susceptibility to chemical-induced mtDNA mutagenesis. We conducted error-corrected, ultra-sensitive Duplex Sequencing to investigate the effects of two known nuclear genome mutagens, cadmium and Aflatoxin B1, on germline mtDNA mutagenesis in Caenorhabditis elegans. Detection of thousands of mtDNA mutations revealed pervasive heteroplasmy in C. elegans and that mtDNA mutagenesis is dominated by C:G → A:T mutations generally attributed to oxidative damage. However, there was no effect of either exposure on mtDNA mutation frequency, spectrum, or trinucleotide context signature despite a significant increase in nuclear mutation rate after aflatoxin B1 exposure. Mitophagy-deficient mutants pink-1 and dct-1 accumulated significantly higher levels of mtDNA damage compared to wild-type C. elegans after exposures. However, there were only small differences in mtDNA mutation frequency, spectrum, or trinucleotide context signature compared to wild-type after 3050 generations, across all treatments. These findings suggest mitochondria harbor additional previously uncharacterized mechanisms that regulate mtDNA mutational processes across generations.