Arachidonic and docosahexanoic acid content of bovine brain myelin: implications for the pathogenesis of multiple sclerosis

Lipids were extracted from bovine brain myelin using a mixture of hexane and isopropanol (3:2). Myelin lipids were resolved, using Sep Pak chromatography, into four fractions: Fraction 1 contained neutral lipids, fraction 2, free fatty acids, fraction 3, ethanolamine phospholipids and fraction 4, ch...

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Bibliographic Details
Published inNeurochemical research Vol. 15; no. 1; p. 7
Main Authors Ansari, K A, Shoeman, D W
Format Journal Article
LanguageEnglish
Published United States 01.01.1990
Subjects
Animals
Arachidonic Acid
Arachidonic Acids - metabolism
Cattle
Chromatography, High Pressure Liquid
Fatty Acids - metabolism
Multiple Sclerosis - metabolism
Myelin Sheath - analysis
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Summary:Lipids were extracted from bovine brain myelin using a mixture of hexane and isopropanol (3:2). Myelin lipids were resolved, using Sep Pak chromatography, into four fractions: Fraction 1 contained neutral lipids, fraction 2, free fatty acids, fraction 3, ethanolamine phospholipids and fraction 4, choline phospholipids. Doscosahexanoic (DHA) and arachidonic (AA) acids in these fractions were measured by RPHPLC. Fraction 2 was analyzed directly, the other three fractions were subjected to alkaline hydrolysis before analysis for DHA and AA. DHA and AA were not found in fraction 1. Both DHA and AA were found in fractions 2 and 3. Only AA was consistently found in fraction 4. These results were confirmed by GC.
ISSN:0364-3190
DOI:10.1007/BF00969177