Lethal and Sublethal Concentrations of Formulated Larvicides Against Susceptible Aedes aegypti

• Published in: Journal of the American Mosquito Control Association Vol. 38; no. 4; pp. 250 - 260
• Main Authors: Aldridge, Robert L, Alto, Barry W, Connelly, C Roxanne, Okech, Bernard, Siegfried, Blair, Linthicum, Kenneth J
• Format: Journal Article
• Language: English
• Published: United States American Mosquito Control Association 01.12.2022
• Subjects: Adults; Aedes; Aedes aegypti; Animals; Aquatic insects; Bacillus thuringiensis; Bio-assays; Bioassays; Body size; Chemical control; Dengue fever; Entomology; Female; Females; Insect control; Insecticides; Insecticides - pharmacology; Larva; Larvae; Larvicides; Male; Males; Mortality; Mosquito Control; Mosquito Vectors; Mosquitoes; Pesticides; Sex; Sex differences; Sublethal effects; Temefos - pharmacology; Vectors; organophosphate; Bacillus thuringiensis israelensis; insect growth regulator; sex ratio; wing length
• ISSN: 8756-971X; 1943-6270
• DOI: 10.2987/22-7084

Chemical control of vectors depends on the effective application of formulated insecticides. In this study we evaluated formulated larvicides using a larval bioassay against susceptible Aedes aegypti. The estimated larvicide lethal concentrations for 50% mortality (LC50s) were 25.7 μg/liter (Natular 2EC), 3.13 μg/liter (Abate 4E), 0.43 μg/liter (Altosid), 0.03 μg/liter (Nyguard), and 500.6 ITU/liter (VectoBac12AS containing Bacillus thuringiensis israelensis). Sublethal effects were identified and documented from adults that survived exposure to these estimated LC50s (body size and sex proportion). We observed changes in net growth as measured by adult wing lengths. For those larvae exposed to estimated LC50s, the average size of adults was between 0.1% and 10.6% smaller for males and between 1.1% and 13.6% smaller for females compared to controls. Sex proportions varied between larvicides, but some were significantly different from the control, favoring greater survival of females than males.