Calcium Transporter 1 and Epithelial Calcium Channel Messenger Ribonucleic Acid Are Differentially Regulated by 1,25 Dihydroxyvitamin D3 in the Intestine and Kidney of Mice

• Published in: Endocrinology (Philadelphia) Vol. 144; no. 9; pp. 3885 - 3894
• Main Authors: Song, Yurong, Peng, Xiaorong, Porta, Angela, Takanaga, Hitomi, Peng, Ji-Bin, Hediger, Matthias A, Fleet, James C, Christakos, Sylvia
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Endocrine Society 01.09.2003
• Subjects: Animal Feed; Animals; Biological and medical sciences; Calbindins; Calcium Channels - genetics; Calcium Channels - metabolism; Calcium, Dietary - pharmacokinetics; Duodenum - metabolism; Enzymes. Coenzymes. Vitamins. Pigments; Female; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation - drug effects; Intestinal Absorption - physiology; Kidney - metabolism; Metabolisms and neurohumoral controls; Mice; Mice, Inbred C57BL; Organ Specificity; Pregnancy; RNA, Messenger - metabolism; S100 Calcium Binding Protein G - genetics; TRPV Cation Channels; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Vertebrates: endocrinology; Vitamin D - analogs & derivatives; Vitamin D - pharmacology; Weaning; Calcium; Digestive system; Rodentia; Gut; Ionic channel; Epithelium; Inorganic element; Kidney; Vertebrata; Mammalia; Urinary system; Mouse; Animal
• ISSN: 0013-7227; 1945-7170
• DOI: 10.1210/en.2003-0314

We examined the expression of calcium transporter 1 (CaT1) and epithelial calcium channel (ECaC) mRNA in the duodenum and kidney of mice. Intestinal CaT1 mRNA level increased 30-fold at weaning, coincident with the induction of calbindin-D9k expression. In contrast, renal CaT1 and ECaC mRNA expression was equal until weaning when ECaC mRNA is induced and CaT1 mRNA levels fall 70%. Long- and short-term adaptation to changes in dietary calcium (Ca) level and 1,25 dihydroxyvitamin D3 [1,25(OH)2D3] injection strongly regulated duodenal calbindin D9k and CaT1 mRNA. Following a single dose of 1,25(OH)2D3, induction of CaT1 mRNA occurred rapidly (within 3 h, peak at 6 h of 9.6 ± 0.8-fold) and preceded the induction of intestinal Ca absorption (significantly increased at 6 h, peak at 9 h). Neither renal CaT1 nor ECaC mRNA were strongly regulated by dietary calcium level or 1,25(OH)2D3 injection. Our data indicate that CaT1 and ECaC mRNA levels are differentially regulated by 1,25(OH)2D3 in kidney and intestine and that there may be a specialized role for CaT1 in kidney in fetal and neonatal development. The rapid induction of intestinal CaT1 mRNA expression by 1,25(OH)2D3, and the marked induction at weaning, suggest that CaT1 is critical for 1,25(OH)2D3-mediated intestinal Ca absorption.