A Flow Cytometry-Based Approach to Unravel Viral Interference with the MHC Class I Antigen Processing and Presentation Pathway

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1988; p. 187
• Main Authors: Praest, Patrique, de Buhr, Hendrik, Wiertz, Emmanuel J H J
• Format: Journal Article
• Language: English
• Published: United States 01.01.2019
• Subjects: Antigen Presentation - immunology; ATP-Binding Cassette Transporters - metabolism; Cell Line, Tumor; Cell Membrane - metabolism; Down-Regulation; Flow Cytometry - methods; Histocompatibility Antigens Class I - immunology; Humans; Lentivirus - metabolism; Peptides - metabolism; Polyethyleneimine - chemistry; Transduction, Genetic; Viruses - immunology; Antigen presentation; Flow cytometry; Transfection; Antigen processing; Lentiviral transduction; Transporter associated with antigen processing (TAP); Cell surface staining; Immune evasion; Viral infection; Major histocompatibility complex (MHC) class I
• ISSN: 1940-6029
• DOI: 10.1007/978-1-4939-9450-2_14

MHC class I molecules are an important component of the cell-mediated immune defense, presenting peptides to surveilling CD8 cytotoxic T cells. During viral infection, MHC class I molecules carry and display viral peptides at the cell surface. CD8 T cells that recognize these peptides will eliminate the virus-infected cells. Viruses counteract this highly sophisticated host detection system by downregulating cell surface expression of MHC class I molecules.In this chapter, we describe a flow cytometry-based method that can be used for the identification of viral gene products potentially responsible for evasion from MHC class I-restricted antigen presentation. The gene(s) of interest are expressed constitutively through lentiviral transduction of cells. Subsequently, MHC I surface expression is monitored using MHC class I-specific antibodies. Once the viral gene product responsible for MHC I downregulation has been identified, the same cells can be used to elucidate the mechanism of action. The stage at which interference with antigen processing occurs can be identified using specific assays. An essential step frequently targeted by viruses is the translocation of peptides into the ER by the transporter associated with antigen processing, TAP. TAP function can be measured using a highly specific in vitro assay involving flow cytometric evaluation of the import of a fluorescent peptide substrate.The protocol described in this chapter enables the identification of virus-encoded MHC class I inhibitors that hinder antigen processing and presentation. Subsequently, their mechanism of action can be unraveled; this knowledge may help to rectify their actions.