Identification of Associated Proteins by Immunoprecipitation and Mass Spectrometry Analysis

Protein-protein interactions play central roles in intercellular and intracellular signal transduction. Impairment of protein-protein interactions causes many diseases such as cancer, cardiomyopathies, diabetes, microbial infections, and genetic and neurodegenerative disorders. Immunoprecipitation i...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1407; p. 131
Main Authors Cao, Xiumei, Yan, Jianshe
Format Journal Article
LanguageEnglish
Published United States 01.01.2016
Subjects
Cells, Cultured
Chromatography, Liquid
Cyclic AMP - metabolism
Dictyostelium - metabolism
Immunoprecipitation
Mass Spectrometry
Protein Interaction Mapping - methods
Tandem Mass Spectrometry
Dictyostelium discoideum
Immunoprecipitation
Protein-protein interaction
Mass spectrometry
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Summary:Protein-protein interactions play central roles in intercellular and intracellular signal transduction. Impairment of protein-protein interactions causes many diseases such as cancer, cardiomyopathies, diabetes, microbial infections, and genetic and neurodegenerative disorders. Immunoprecipitation is a technique in which a target protein of interest bound by an antibody is used to pull down the protein complex out of cell lysates, which can be identified by mass spectrometry. Here, we describe the protocol to immunoprecipitate and identify the components of the protein complexes of ElmoE in Dictyostelium discoideum cells.
ISSN:1940-6029
DOI:10.1007/978-1-4939-3480-5_10