A method for determination of one hundred endogenous steroids in human serum by gas chromatography-tandem mass spectrometry

Steroid profiling helps various pathologies to be rapidly diagnosed. Results from analyses investigating steroidogenic pathways may be used as a tool for uncovering pathology causations and proposals of new therapeutic approaches. The purpose of this study was to address still underutilized applicat...

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Published inPhysiological research Vol. 68; no. 2; pp. 179 - 207
Main Authors Hill, M, Hána, Jr, V, Velíková, M, Pařízek, A, Kolátorová, L, Vítků, J, Škodová, T, Šimková, M, Šimják, P, Kancheva, R, Koucký, M, Kokrdová, Z, Adamcová, K, Černý, A, Hájek, Z, Dušková, M, Bulant, J, Stárka, L
Format Journal Article
LanguageEnglish
Published Czech Republic Institute of Physiology 30.04.2019
Subjects
Alzheimer's disease
Chromatography
Cord blood
Corticosteroids
Drug testing
Endocrinology
Gas chromatography
Hormones
Immunomodulation
Ions
Mass spectrometry
Mass spectroscopy
Metabolites
Neurosteroids
Nuclear receptors
Pregnancy
Scientific imaging
Steroid hormones
Steroids
Studies
Umbilical cord
Urine
Womens health
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Summary:Steroid profiling helps various pathologies to be rapidly diagnosed. Results from analyses investigating steroidogenic pathways may be used as a tool for uncovering pathology causations and proposals of new therapeutic approaches. The purpose of this study was to address still underutilized application of the advanced GC-MS/MS platform for the multicomponent quantification of endogenous steroids. We developed and validated a GC-MS/MS method for the quantification of 58 unconjugated steroids and 42 polar conjugates of steroids (after hydrolysis) in human blood. The present method was validated not only for blood of men and non-pregnant women but also for blood of pregnant women and for mixed umbilical cord blood. The spectrum of analytes includes common hormones operating via nuclear receptors as well as other bioactive substances like immunomodulatory and neuroactive steroids. Our present results are comparable with those from our previously published GC-MS method as well as the results of others. The present method was extended for corticoids and 17alpha-hydroxylated 5alpha/ß-reduced pregnanes, which are useful for the investigation of alternative "backdoor" pathway. When comparing the analytical characteristics of the present and previous method, the first exhibit by far higher selectivity, and generally higher sensitivity and better precision particularly for 17alpha-hydroxysteroids.
ISSN:0862-8408
1802-9973
DOI:10.33549/physiolres.934124