Regulation of cyclic GMP phosphodiesterase in the submandibular gland of adult rat

Cyclic GMP phosphodiesterase was investigated in the submandibular gland of the adult rat to elucidate the regulatory mechanisms of cGMP concentration in this gland. Ca2+ sensitivity was easily demonstrated as in other tissues using EGTA in the buffer for elution from DEAE-cellulose. The presence of...

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Bibliographic Details
Published inCanadian journal of physiology and pharmacology Vol. 61; no. 2; p. 109
Main Authors Yokoyama, N, Murota, Y, Negishi, T, Saito, M, Furuyama, S
Format Journal Article
LanguageEnglish
Published Canada 01.02.1983
Subjects
3',5'-Cyclic-GMP Phosphodiesterases - analysis
3',5'-Cyclic-GMP Phosphodiesterases - antagonists & inhibitors
Animals
Calcium - pharmacology
Calmodulin - pharmacology
Chromatography, DEAE-Cellulose
Enzyme Activation
Kinetics
Male
Rats
Rats, Inbred Strains
Submandibular Gland - enzymology
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Summary:Cyclic GMP phosphodiesterase was investigated in the submandibular gland of the adult rat to elucidate the regulatory mechanisms of cGMP concentration in this gland. Ca2+ sensitivity was easily demonstrated as in other tissues using EGTA in the buffer for elution from DEAE-cellulose. The presence of inhibitor proteins for basal and calmodulin-activated portions of Ca2+-dependent phosphodiesterase was suggested. The inhibitor for the basal activity of Ca2+-dependent phosphodiesterase was deemed to be a heat-labile protein, which decreased Vmax, but had no effect on the Km value for cGMP. The presence of more than one kind of inhibitor for the calmodulin-activated portion of the Ca2+-dependent phosphodiesterase was also suggested. One of these, which was not absorbed on DEAE-cellulose, was a heat-labile protein which caused an increase of Km for cGMP, but no change of Vmax.
ISSN:0008-4212
1205-7541
DOI:10.1139/y83-015