Micrococcus luteus, a bacterium with a high genomic G + C content, contains Escherichia coli-type promoters

The G + C content of Micrococcus luteus DNA is 74%, which is much higher than that of Escherichia coli (about 50%). In order to understand the influence of GC-directed mutation pressure (GC pressure) on the promoter structure, the initiation sites for both in vitro and in vivo transcription of M. lu...

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Bibliographic Details
Published inMolecular & general genetics Vol. 218; no. 3; p. 384
Main Authors Nakayama, M, Fujita, N, Ohama, T, Osawa, S, Ishihama, A
Format Journal Article
LanguageEnglish
Published Germany 01.09.1989
Subjects
Base Sequence
Electrophoresis, Polyacrylamide Gel
Micrococcus - genetics
Molecular Sequence Data
Mutation
Operon
Promoter Regions, Genetic
Restriction Mapping
RNA-Directed DNA Polymerase
Sequence Homology, Nucleic Acid
Spectinomycin - analysis
Streptomycin - analysis
Transcription, Genetic
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Summary:The G + C content of Micrococcus luteus DNA is 74%, which is much higher than that of Escherichia coli (about 50%). In order to understand the influence of GC-directed mutation pressure (GC pressure) on the promoter structure, the initiation sites for both in vitro and in vivo transcription of M. luteus streptomycin (str) and spectinomycin (spc) operons were identified by the reverse transcriptase mapping method. The promoter sequences of M. luteus are similar to those of E. coli, but have significantly higher G + C contents. In an in vitro run-off transcription assay using truncated DNA templates, RNA polymerases from both M. luteus and E. coli were able to transcribe correctly from both the str promoter and the spc major promoter of M. luteus.
ISSN:0026-8925
DOI:10.1007/BF00332399