Plate-Based Measurement of Respiration by Isolated Mitochondria

Measuring respiration rate can be a powerful way to assess energetic function in isolated mitochondria. Current, plate-based methods have several advantages over older, suspension-based systems, including greater throughput and the requirement of only μg quantities of material. In this chapter, we d...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1782; p. 301
Main Authors Mookerjee, Shona A, Quinlan, Casey L, Wong, Hoi-Shan, Dighe, Pratiksha, Brand, Martin D
Format Journal Article
LanguageEnglish
Published United States 01.01.2018
Subjects
Animals
Cell Respiration
Fluorometry - instrumentation
Fluorometry - methods
Mitochondria, Muscle - metabolism
Oxygen Consumption
Rats
Rats, Wistar
Mitochondrial respiration
Oxygen consumption
Electron flow
Respiratory control ratio
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Summary:Measuring respiration rate can be a powerful way to assess energetic function in isolated mitochondria. Current, plate-based methods have several advantages over older, suspension-based systems, including greater throughput and the requirement of only μg quantities of material. In this chapter, we describe a plate-based method for measuring oxygen consumption by isolated adherent mitochondria.
ISSN:1940-6029
DOI:10.1007/978-1-4939-7831-1_17