Prediction of size-selective permitted daily exposures for mineral oil mist based on an in vitro study in different scenarios

• Published in: International journal of environmental health research Vol. 34; no. 2; pp. 1044 - 1052
• Main Authors: Moradpour, Zahra, Zendehdel, Rezvan, Hajipour-Verdom, Behnam, Abdolmaleki, Parviz, Khavanin, Ali, Vahabi Shekarloo, Masoomeh
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.02.2024; Taylor & Francis LLC
• Subjects: A549 cell line; benchmark dose; Benchmarks; Bioassays; Body size; Cell lines; Comet assay; Confidence limits; Damage prevention; Deoxyribonucleic acid; DNA; DNA damage; Exposure; Genotoxicity; Human populations; Injury prevention; Lower bounds; Metalworking fluids; Mineral oils; Occupational exposure; Oil mist; permitted daily exposure; Upper bounds; benchmark dose; Metalworking fluids; A549 cell line; permitted daily exposure
• ISSN: 0960-3123; 1369-1619
• DOI: 10.1080/09603123.2023.2196059

The incidence of DNA damage from exposure to specific types of metalworking fluids has been reported. In this research, size-selective permissible limits to prevent genotoxic damage in A549 cell lines exposed to two types of mineral oil were estimated for the first time using a benchmark dose approach and extrapolated to workers. The comet assay was performed based on Olive and Banath protocol to determine DNA damage. Then, the Benchmark Dose, the 95% lower bound confidence limit BMD, and the 95% upper-bound confidence limit BMD were determined using continuous response data. Finally, the four Benchmark Dose levels reported in the A549 cell line were extrapolated to the human population in occupational settings in two phases. This study showed when determining the permissible limits, the type used or unused, the type of injury, the organ affected in the body and the size of the particles should also be considered.