Prominent toxicity of isocyanates and maleic anhydrides to Caenorhabditis elegans: Multilevel assay for typical organic additives of biodegradable plastics

• Published in: Journal of hazardous materials Vol. 442; p. 130051
• Main Authors: Li, Xinyu, Chen, Yingxin, Gao, Wei, Mo, Aoyun, Zhang, Yalin, Jiang, Jie, He, Defu
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.01.2023
• Subjects: Biodegradable plastics; Caenorhabditis elegans; Isocyanates; Organic additives; Soil; Isocyanates; Soil; Caenorhabditis elegans; Biodegradable plastics; Organic additives
• ISSN: 0304-3894; 1873-3336
• DOI: 10.1016/j.jhazmat.2022.130051

Biodegradable plastics (BDP) are increasingly applied; however, there has been of concerns about their environmental safety, especially from nondegradable additive compositions. Until now, data of ecotoxicity of BDP additives is scarce. Here, nematode C. elegans was used to comparatively evaluate toxicity of an isocyanate additive, i.e., Hexamethylene diisocyanate (HDI), a maleic anhydride, i.e., Diallyl maleate (DIM), and other four BDP organic additives. These additives caused lethality of nematodes at µg L-1 level, of lowest LC50 value of HDI/DIM. Uniform exposure to these additives resulted in various degrees of inhibitions in body volumes and longevity, indicating developmental toxicity. Moreover, BDP additives induced significant elevations of gst-4 expression, especially mean 123.54 %/234.29 % increase in HDI/DIM group, but reduced ges-1 expression, which indicates oxidative damages and mitochondrial dysfunction. BDP additives further caused inhibition in locomotor and food intake/excretion behavior, and related damages of glutamatergic neurons and GABAergic neurons, indicating their neurotoxicity. We found HDI and DIM presented relatively strong effects on susceptible endpoints including lethality, gst-4, mean lifespan, food intake and excretion behavior. Overall, this study suggests prominent ecotoxic risk of isocyanates and maleic anhydrides as BDP additives, which is significant for the selection of environmentally friendly BDP additives. [Display omitted] •Six biodegradable plastic (BDP) additives caused lethality of C. elegans at µg L-1 level.•BDP additives increased gst-4, but reduced ges-1 expression and lifespan, of various degrees.•BDP additives caused inhibition in locomotor and food intake/excretion behavior.•BDP additives induced damages in glutamatergic neurons and GABAergic neurons.•Isocyanates/maleic anhydrides showed strong toxicity compared to other BDP additives.