Common antigenic properties of a g-type (goose) and a c-type (duck) egg white lysozyme: antibody responses in rabbits and mice

Embden goose (GEWL) and Barbary duck (DEWL) egg white lysozymes possess different amino acid sequences corresponding to the g-type and c-type, respectively. GEWL was shown to be a better immunogen than DEWL in both rabbits and mice. The antigenicity of the two lysozymes was tested using different te...

Full description

Saved in:
Bibliographic Details
Published inExperientia Vol. 48; no. 1; p. 79
Main Authors Hemmen, F, Mahana, W, Jollès, P, Paraf, A
Format Journal Article
LanguageEnglish
Published Switzerland 15.01.1992
Subjects
Animals
Antibody Formation
Antigens - immunology
Binding Sites, Antibody
Binding, Competitive
Ducks
Egg White - analysis
Enzyme-Linked Immunosorbent Assay
Female
Geese
Immune Sera - analysis
Mice
Mice, Inbred BALB C
Muramidase - immunology
Rabbits
Online AccessGet more information

Cover

More Information
Summary:Embden goose (GEWL) and Barbary duck (DEWL) egg white lysozymes possess different amino acid sequences corresponding to the g-type and c-type, respectively. GEWL was shown to be a better immunogen than DEWL in both rabbits and mice. The antigenicity of the two lysozymes was tested using different techniques (i.e. indirect ELISA, inhibition tests and immunoabsorption experiments). Injection of either GEWL or DEWL into rabbits and mice induced both specific antibodies and cross-reacting antibodies. Moreover, anti-GEWL antibodies, in contrast to anti-DEWL antibodies, did not cross-react with hen egg white lysozyme (HEWL), a c-type lysozyme. While the structure of GEWL was not modified after binding to plastic, DEWL was denaturated, but it did keep some native epitopes. It was concluded that g-type and c-type lysozymes, which have different amino acid sequences, exhibit strong common antigenic properties.
ISSN:0014-4754
DOI:10.1007/BF01923613