Live Metabolic Profile Analysis of Zebrafish Embryos Using a Seahorse XF 24 Extracellular Flux Analyzer

The Agilent Seahorse Extracellular Flux Analyzer can be used to measure the oxygen consumption rate (OCR) and extra cellular acidification rate (ECAR), from which mitochondrial bioenergetics measurements can be determined including basal respiration, respiration due to ATP turnover, uncoupled respir...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1797; p. 393
Main Authors Bond, Simon T, McEwen, Kevin A, Yoganantharajah, Prusothman, Gibert, Yann
Format Journal Article
LanguageEnglish
Published United States 2018
Subjects
Embryo
Mitochondria
Seahorse
Development
Zebrafish
Metabolism
Respiration
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Summary:The Agilent Seahorse Extracellular Flux Analyzer can be used to measure the oxygen consumption rate (OCR) and extra cellular acidification rate (ECAR), from which mitochondrial bioenergetics measurements can be determined including basal respiration, respiration due to ATP turnover, uncoupled respiration/proton leak, and maximum respiration. This novel method demonstrates how to use a Seahorse XF 24 Extracellular Flux Analyzer to measure the bioenergetic flux of zebrafish embryos in vivo during development. This provides a tool that enables characterization of metabolic parameters in a living organism, utilizing Agilent Islet Capture Microplates where respiration parameters can be compared between controls and genetically altered/pharmacologically treated embryos in real time. This method can be used to analyze and identify novel pharmaceuticals and genes that influence respiration, mitochondrial function, and metabolism.
ISSN:1940-6029
DOI:10.1007/978-1-4939-7883-0_21