Blue-sensitive cones in the primate retina: microspectrophotometry of the visual pigment

Direct absorbance and bleaching absorbance-difference spectra were obtained using a photon-counting microspectrophotometer from the outer segments of ten blue-sensitive cones of macaque monkeys. The peak wavelength (lambda max) of the direct measurements was 426 +/- 3.4 nm, whereas the lambda max of...

Full description

Saved in:
Bibliographic Details
Published inExperimental brain research Vol. 56; no. 2; p. 389
Main Authors Mansfield, R J, Levine, J S, Lipetz, L E, Collins, B A, Raymond, G, MacNichol, Jr, E F
Format Journal Article
LanguageEnglish
Published Germany 01.01.1984
Subjects
Animals
Macaca fascicularis
Macaca mulatta
Photoreceptor Cells - analysis
Retinal Pigments - analysis
Spectrophotometry
Online AccessGet more information

Cover

More Information
Summary:Direct absorbance and bleaching absorbance-difference spectra were obtained using a photon-counting microspectrophotometer from the outer segments of ten blue-sensitive cones of macaque monkeys. The peak wavelength (lambda max) of the direct measurements was 426 +/- 3.4 nm, whereas the lambda max of the bleaching difference was 434 +/- 6.6 nm. We consider these values to be upper and lower bounds since both measurements may be shifted in opposite directions by wavelength-dependent effects. Therefore, the true peak sensitivity must be close to 430 nm.
ISSN:0014-4819
DOI:10.1007/BF00236296