Surface effects on PCR reactions in multichip microfluidic platforms

We evaluated the compatibility of several common plastics, commercially available plastic tubing and disposable syringes which might be useful in the construction of microfluidic platforms with respect to the polymerase chain reaction (PCR). A simple and inexpensive plastic test module was construct...

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Bibliographic Details
Published inBiomedical microdevices Vol. 6; no. 1; pp. 75 - 80
Main Authors Panaro, Nicholas J, Lou, Xing Jian, Fortina, Paolo, Kricka, Larry J, Wilding, Peter
Format Journal Article
LanguageEnglish
Published United States Springer Nature B.V 01.03.2004
Subjects
Equipment Design
Equipment Failure Analysis - methods
Materials Testing - methods
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Plastics - chemistry
Polymerase Chain Reaction - instrumentation
Polymerase Chain Reaction - methods
Surface Properties
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Summary:We evaluated the compatibility of several common plastics, commercially available plastic tubing and disposable syringes which might be useful in the construction of microfluidic platforms with respect to the polymerase chain reaction (PCR). A simple and inexpensive plastic test module was constructed in order to evaluate some of the construction plastics. We also investigated the effect of addition of PEG 8000 to PCR reaction mixtures on the compatibility of materials. These studies identified several common plastics, plastic tubing, and disposable syringes which were compatible with the PCR reaction.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1387-2176
1572-8781
DOI:10.1023/B:BMMD.0000013369.34716.8d