Simple and Sensitive Determination of Metformin in Human Plasma Using an Ion-Pair LC Method

• Published in: Chromatographia Vol. 70; no. 9-10; pp. 1511 - 1514
• Main Authors: Liu, Qing-Feng, Li, Zhong-Dong, Shi, Xiao-Jin, Jiao, Zheng, Zhong, Ming-Kang
• Format: Journal Article
• Language: English
• Published: Wiesbaden Wiesbaden : Vieweg Verlag 01.11.2009; Vieweg Verlag; Springer
• Subjects: Analysis; Analytical Chemistry; Biological and medical sciences; Chemistry; Chemistry and Materials Science; Chromatography; General pharmacology; Laboratory Medicine; Limited Short Communication; Medical sciences; Pharmacology. Drug treatments; Pharmacy; Proteomics; Ion-pair column liquid chromatography; Metformin analysis; Human plasma; Human; Biological fluid; Chemical analysis; Ion pair; HPLC chromatography; Mobile phase; Ion interaction chromatography; Blood; Blood plasma; Reversed phase chromatography; Hypoglycemic agent; Biguanides; Ultraviolet detector; Metformin; Quantitative analysis
• ISSN: 0009-5893; 1612-1112
• DOI: 10.1365/s10337-009-1339-x

A simple, selective and sensitive ion-pair liquid chromatography method was described for the determination of metformin in human plasma. Plasma samples (200 μL) were deproteinated with 10% (v/v) perchloric acid followed by LC analysis using a Symmetry C₁₈ column and a mobile phase of 29% acetonitrile—71% 2 mM sodium dodecyl sulfate (with 350 μL 0.1 M HCl) operated at a flow rate of 1.5 mL min⁻¹. Metformin and the internal standard (sodium phenytoin) were detected at 232 nm and eluted at 6.8 and 7.9 min, respectively. Calibration curves were linear (r > 0.9990) between 9 and 2,000 ng mL⁻¹. The quantitation limit was 9 ng mL⁻¹, intra- and inter-day precision (CV) were 5.26% or less, and accuracy values were found to be within 5.95% of the nominal concentration.