Natural size classes in DNA from Chinese hamster metaphase chromosomes

DNA from isolated Chinese hamster ovary (CHO) metaphase chromosomes can be obtained in three different molecular weight classes. The two largest forms have sedimentation coefficients of 80 and 120 S at 7,500 rpm. Based on sedimentation and speed dependence analysis these have molecular weights of 22...

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Bibliographic Details
Published inChromosoma Vol. 95; no. 1; p. 44
Main Authors Murata-Collins, J L, Clark, R W
Format Journal Article
LanguageEnglish
Published Austria 01.01.1987
Subjects
Animals
Cell Line
Chromosomes - analysis
Cricetinae
Cricetulus
DNA - isolation & purification
Female
Hydrogen-Ion Concentration
Metaphase
Molecular Weight
Ovary
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Summary:DNA from isolated Chinese hamster ovary (CHO) metaphase chromosomes can be obtained in three different molecular weight classes. The two largest forms have sedimentation coefficients of 80 and 120 S at 7,500 rpm. Based on sedimentation and speed dependence analysis these have molecular weights of 220 million and above 5,000 million, and are thought to be analogs of DNA classes observed in a prior study of human metaphase chromosomes. An extract can be converted to primarily the 80 S form through alkaline pH treatment of metaphase DNA. The third class (45 S DNA) is formed as a result of metaphase chromosome exposure to the nuclease Bal31, and has a mass distribution analogous to the CHO replicon.
ISSN:0009-5915
DOI:10.1007/BF00293840