Sacha inchi (Plukenetia volubilis L.) oil composition varies with changes in temperature and pressure in subcritical extraction with n-propane

• Published in: Industrial crops and products Vol. 87; pp. 64 - 70
• Main Authors: Zanqui, Ana Beatriz, da Silva, Claudia Marques, de Morais, Damila Rodrigues, Santos, Jandyson Machado, Ribeiro, Suellen Andressa Oenning, Eberlin, Marcos Nogueira, Cardozo-Filho, Lúcio, Visentainer, Jesuí Vergílio, Gomes, Sandra Terezinha Marques, Matsushita, Makoto
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.2016
• Subjects: Alpha linoleic acid; Easy ambient sonic-spray ionization; Omega 3; Phytosterols; Plukenetia volubilis L; Subcritical fluid extraction; Palmitic acid (PubChem CID: 985); MUFA; Plukenetia volubilis L; Alpha linoleic acid; SFA; HDL; SE; S; Vaccenic acid (PubChem CID: 5281127); SubFE; Cholestane (PubChem CID: 6857534); Omega 3; α-Linolenic acid (PubChem CID: 5280934); Easy ambient sonic-spray ionization; OL; Sitosterol (PubChem CID: 222284); PUFA; Stigmasterol (PubChem CID: 5280794); Stearic acid (PubChem CID: 5281); Phytosterols; PCA; Subcritical fluid extraction; LA; ALA; Oleic acid (PubChem CID: 445639); TL; FA; TAG; Linoleic acid (PubChem CID: 5280450)
• ISSN: 0926-6690; 1872-633X
• DOI: 10.1016/j.indcrop.2016.04.029

[Display omitted] •Sacha inchi oil was obtained via subcritical n-propane extraction.•An average of 442mgg−1 of alpha linolenic acid in oil was obtained.•Higher temperatures and pressures increase the phytosterols content.•The α-linolenic acid was the predominant triacylglycerol in Sacha oil. Sacha inchi is an oilseed crop that shows high oil content and it is an excellent source of polyunsaturated fatty acids. Temperature and pressure can influence in lipid composition on the subcritical extraction with n-propane. The highest extraction yield obtained was 30% under the conditions of 60°C and 12MPa. The extracted oil presented 442mg of fatty acid g−1 in terms of alpha linolenic acid. Analysis by easy ambient sonic-spray ionization mass spectrometry showed a typical TAG (Triacylglycerols) profile for Sacha inchi oil with major ions of m/z (mass-to-charge ratio) 895 (ALA-ALA-ALA), 897 (ALA-ALA-LA), 899 (ALA-LA-LA) and 901 (LA-LA-LA or OL-LA-ALA) in a form of adduct of [TAG+Na]+, in which ALA is alfa linoleic acid, LA is linoleic acid and OL is oleic acid. The phytosterols content (86.39–101.92mg 100g−1 of total lipids) varied according to the conditions applied for the n-propane extraction. In general, the subcritical fluid extraction with n-propane was found to preserve the important Sacha inchi oil bioactive constituents, that is, fatty acids, triacylglycerols and phytosterols. Regression analysis also showed that higher temperatures and pressures result in higher yields of phytosterols.