A novel hybrid platform for the preparation of disposable enzyme biosensors based on poly(3,4-ethylenedioxythiophene) electrodeposition in an ionic liquid medium onto gold nanoparticles-modified screen-printed electrodes

• Published in: Journal of electroanalytical chemistry (Lausanne, Switzerland) Vol. 656; no. 1; pp. 152 - 158
• Main Authors: SERAFIN, V, AGÜI, L, YANEZ-SEDENO, P, PINGARRON, J. M
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 15.06.2011; Elsevier
• Subjects: Alcohol dehydrogenase; Beers; Biological and medical sciences; Biosensors; Biotechnology; Chemistry; Electrochemistry; Electrodes: preparations and properties; Exact sciences and technology; Fermented food industries; Food industries; Fundamental and applied biological sciences. Psychology; General and physical chemistry; Gold nanoparticles-modified screen-printed electrodes; Ion selective electrodes; Ionic liquids; Methods. Procedures. Technologies; NADH; Poly(3,4-ethylenedioxythiophene); Tyrosinase; Various methods and equipments; Gold nanoparticles-modified screen-printed electrodes; Ionic liquids; NADH; Alcohol dehydrogenase; Poly(3,4-ethylenedioxythiophene); Tyrosinase; Chemical analysis; Nanoparticle; Screen-printed electrode; Alcohol; Electrochemical polymerization; Beer; Ionic liquid; Thiophene derivative polymer; Amperometry; Electrochemical reaction; Modified material; Disposable; Gold; Purine nucleotide; Ethanol; Enzyme; Transition metal; Monophenol monooxygenase; Carbon; Enzyme electrode; Electrochemical detector; Conducting polymers; Phenol; Biosensor; Preparation; Oxidoreductases; Immobilized enzyme
• ISSN: 1572-6657; 1873-2569
• DOI: 10.1016/j.jelechem.2010.11.038

A novel electrochemical platform for the preparation of disposable enzyme biosensors is reported in this work. This platform is constructed by electrodeposition of poly(3,4-ethylenedioxythiophene) (PEDOT) using the ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate (BMIMPF 6) as the electropolymerization solvent onto gold nanoparticles-modified screen-printed carbon electrodes (SPCE). The enzymes alcohol dehydrogenase (ADH) or Tyrosinase were entrapped onto the electrode surface during the electropolymerization step. The potentiostatic electropolymerization process of PEDOT on gold nanoparticles-modified SPCE was optimized and the resulting modified electrodes characterized voltammetrically and by electrochemical impedance spectroscopy (EIS). The NADH amperometric detection at PEDOT/nAu/SPCE was also optimized and compared with that produced at a PEDOT/SPCE. ADH/PEDOT/nAu/SPCEs were constructed. The measured current for ethanol was 30% larger than that obtained using ADH/PEDOT/SPCEs. At a detection potential of +300 mV, a calibration graph for ethanol with a linear range between 5 and 100 μM was obtained with a detection limit of 2 μM. The PEDOT/nAu/SPCEs electrodes were also tested for the preparation of Tyrosinase biosensors. Using a detection potential of −150 mV, a linear calibration graph for phenol was constructed over the 0.1–50 μM concentration range, with a limit of detection of 0.02 μM.