The influx of uric acid and other purines into everted jejunal sacs of the rat and hamster

The in vitro transport of [2-14-C]uric acid, [8-14-C]hypoxanthine, and [8-14-C]xanthine, each dissolved in Krebs--Ringer bicarbonate buffer, was studied with everted jejunal sacs from rat and hamster. No evidence could be obtained for the development of a concentration gradient between the intracell...

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Bibliographic Details
Published inCanadian journal of physiology and pharmacology Vol. 53; no. 1; p. 113
Main Authors Khan, A H, Wilson, S, Crawhall, J C
Format Journal Article
LanguageEnglish
Published Canada 01.02.1975
Subjects
Allopurinol - pharmacology
Anaerobiosis
Animals
Biological Transport
Cricetinae
Dinitrophenols - pharmacology
Female
Hypoxanthines - metabolism
In Vitro Techniques
Jejunum - metabolism
Purines - metabolism
Rats
Time Factors
Uric Acid - metabolism
Xanthines - metabolism
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Summary:The in vitro transport of [2-14-C]uric acid, [8-14-C]hypoxanthine, and [8-14-C]xanthine, each dissolved in Krebs--Ringer bicarbonate buffer, was studied with everted jejunal sacs from rat and hamster. No evidence could be obtained for the development of a concentration gradient between the intracellular fluid and the incubation medium or between the sac contents and the incubation medium, for any of the three oxypurines. Inhibitiors of active transport, such as anaerobiosis for dinitrophenol, had no significant effect on the rate of transport. A large percentage of hypoxanthine and xanthine was oxidized to urine acid in the sac-wall homogenate, sac contents, and incubation medium during the course of the incubation. This oxidation could be prevented by addition of allopurinol (3 mM) to the incubation medium, but concentration gradients were still not obtained. No active transport mechanism could be demonstrated for uric acid, hypoxanthine, or xanthine in rat or hamster jejunum.
ISSN:0008-4212
DOI:10.1139/y75-015