Monitoring Functional Responses to Hypoxia in Single Carotid Body Cells

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 1742; p. 125
• Main Authors: Muñoz-Cabello, Ana María, Torres-Torrelo, Hortensia, Arias-Mayenco, Ignacio, Ortega-Sáenz, Patricia, López-Barneo, José
• Format: Journal Article
• Language: English
• Published: United States 01.01.2018
• Subjects: Animals; Carotid Body - cytology; Carotid Body - physiology; Cell Hypoxia; Cells, Cultured; Chemoreceptor Cells - metabolism; Cytophotometry; Mice; Patch-Clamp Techniques - methods; Rats; Single-Cell Analysis - methods; Microfluorimetry; Cytosolic calcium; O2-sensitive ion channels; Enzymatic cell dispersion; Patch clamp; Hypoxia; Ionic currents; Acute oxygen sensing; Glomus cells

The carotid body is the main arterial chemoreceptor in mammals that mediates the cardiorespiratory reflexes activated by acute hypoxia. Here we describe the protocols followed in our laboratory to study responsiveness to hypoxia of single, enzymatically dispersed, glomus cells monitored by microfluo...