Monitoring Functional Responses to Hypoxia in Single Carotid Body Cells

The carotid body is the main arterial chemoreceptor in mammals that mediates the cardiorespiratory reflexes activated by acute hypoxia. Here we describe the protocols followed in our laboratory to study responsiveness to hypoxia of single, enzymatically dispersed, glomus cells monitored by microfluo...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1742; p. 125
Main Authors Muñoz-Cabello, Ana María, Torres-Torrelo, Hortensia, Arias-Mayenco, Ignacio, Ortega-Sáenz, Patricia, López-Barneo, José
Format Journal Article
LanguageEnglish
Published United States 01.01.2018
Subjects
Animals
Carotid Body - cytology
Carotid Body - physiology
Cell Hypoxia
Cells, Cultured
Chemoreceptor Cells - metabolism
Cytophotometry
Mice
Patch-Clamp Techniques - methods
Rats
Single-Cell Analysis - methods
Microfluorimetry
Cytosolic calcium
O2-sensitive ion channels
Enzymatic cell dispersion
Patch clamp
Hypoxia
Ionic currents
Acute oxygen sensing
Glomus cells
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Summary:The carotid body is the main arterial chemoreceptor in mammals that mediates the cardiorespiratory reflexes activated by acute hypoxia. Here we describe the protocols followed in our laboratory to study responsiveness to hypoxia of single, enzymatically dispersed, glomus cells monitored by microfluorimetry and the patch-clamp technique.
ISSN:1940-6029
DOI:10.1007/978-1-4939-7665-2_12