Cellular immune response of the Asian corn borer, Ostrinia furnacalis (Lepidoptera: Pyralidae), to infection by the entomopathogenic fungus, Beauveria bassiana

• Published in: European journal of entomology Vol. 113; no. 1; pp. 415 - 422
• Main Authors: SHEN, Dongxu, LI, Miao, CHU, Yuan, LANG, Minglin, AN, Chunju
• Format: Journal Article
• Language: English
• Published: Institute of Entomology, Biology Centre, Czech Academy of Science 01.01.2016
• Subjects: beauveria bassiana; cellular immune response; entomopathogenic fungus; haemocyte; lepidoptera; nodulation; ostrinia furnacalis; phagocytosis; pyralidae
• ISSN: 1210-5759; 1802-8829
• DOI: 10.14411/eje.2016.054

The term cellular immune response refers to haemocyte-mediated responses, including phagocytosis, nodulation, and encapsulation. In the present study, we identified five types of circulating haemocytes in larvae of the haemolymph of the Asian corn borer, Ostrinia furnacalis (Guenée), including granulocytes, oenocytoids, plasmatocytes, prohaemocytes, and spherulocytes. The relative number of total free haemocytes per larva decreased significantly 0.5, 24, and 36 h after the injection of Beauveria bassiana conidia. Upon conidia challenge, both phagocytosis and nodulation were observed in the collected haemolymph from O. furnacalis larvae. In addition, plasma was found to be necessary for both phagocytosis and nodulation. Therefore, we here confirm that phagocytosis and nodulation are involved in O. funacalis larvae during their fight against infection by B. bassiana, and further, that the cellular immune response of O. furnacalis helps eliminate the invading organisms despite the fact that not all the fungal conidia are killed.