Telomeric sequences derived from laser-microdissected polytene chromosomes

Telomeric fragments from salivary gland squashes of Drosophila melanogaster Oregon R. were produced by a new microdissection technique, UV laser microbeam dissection. Microdissection, an essential step in microcloning procedures, is usually performed using micromanipulators and microneedles. Recentl...

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Bibliographic Details
Published inChromosoma Vol. 98; no. 5; p. 351
Main Authors Ponelies, N, Bautz, E K, Monajembashi, S, Wolfrum, J, Greulich, K O
Format Journal Article
LanguageEnglish
Published Austria 01.11.1989
Subjects
Animals
Blotting, Southern
Chromosomes - ultrastructure
Cloning, Molecular
DNA - isolation & purification
Drosophila melanogaster - genetics
Fluorescent Antibody Technique
Humans
Lasers
Microscopy, Electron, Scanning
Repetitive Sequences, Nucleic Acid
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Summary:Telomeric fragments from salivary gland squashes of Drosophila melanogaster Oregon R. were produced by a new microdissection technique, UV laser microbeam dissection. Microdissection, an essential step in microcloning procedures, is usually performed using micromanipulators and microneedles. Recently it has been shown that microdissection can be improved to very high precision if a laser coupled into a microscope is used. A laser microbeam, generated by an excimer pumped dye laser, allows chromosomes to be cut into slices of less than 0.5 micron. Here it is shown, that single copy DNA probes prepared from Drosophila chromosomes by laser microdissection and microcloning relocalize to the chromosomal regions from which they are derived. The combination of laser technique and microcloning provides an advantageous approach for rapid genetic analysis with potential for the study of genetic diseases and genome mapping.
ISSN:0009-5915
DOI:10.1007/BF00292388