Gene Delivery for the Generation of Bioartificial Pacemaker

Electronic pacemakers have been used in patients with heart rhythm disorders for device-supported pacing. While effective, there are such shortcomings as limited battery life, permanent implantation of catheters, the lack of autonomic neurohumoral responses, and risks of lead dislodging. Here we des...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1521; p. 293
Main Authors Chan, Patrick K W, Li, Ronald A
Format Journal Article
LanguageEnglish
Published United States 2017
Subjects
Animals
Dependovirus - metabolism
Fluoroscopy
Gene Transfer Techniques
Green Fluorescent Proteins - metabolism
HEK293 Cells
Humans
Pacemaker, Artificial
Sus scrofa
Adeno-associated virus
Heart rhythms
Gene transfer
Adenovirus
Complete heart block
Bioartificial pacemaker
Hyperpolarization-activated cyclic nucleotide-gated (HCN) channel
Sick sinus syndrome
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Summary:Electronic pacemakers have been used in patients with heart rhythm disorders for device-supported pacing. While effective, there are such shortcomings as limited battery life, permanent implantation of catheters, the lack of autonomic neurohumoral responses, and risks of lead dislodging. Here we describe protocols for establishing porcine models of sick sinus syndrome and complete heart block, and the generation of bioartificial pacemaker by delivering a strategically engineered form of hyperpolarization-activated cyclic nucleotide-gated pacemaker channel protein via somatic gene transfer to convert atrial or ventricular muscle cardiomyocytes into nodal-like cells that rhythmically fire action potentials.
ISSN:1940-6029
DOI:10.1007/978-1-4939-6588-5_21