Detect early stage lung cancer by a LAMP microfluidic chip system with a real-time fluorescent filter processor

This work presents a promising clinical molecular diagnostics for early stage lung cancer. This novel diagnostic method utilized the loopmediated isothermal amplification （LAMP）, micro fluidic chips and a confocal optical detector with a nonlinear fluorescent filter processor. An isothermal amplific...

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Bibliographic Details
Published inScience China. Chemistry Vol. 55; no. 4; pp. 508 - 514
Main Authors Wang, TongZhou, Zhang, Ye, Huang, GuoLiang, Wang, Can, Xie, Lan, Ma, Li, Li, ZhiYong, Luo, XianBo, Tian, Hao, Li, Qiang, Li, Xin, Lv, ZhouYan, Bao, XiaoFan
Format Journal Article
LanguageEnglish
Published Heidelberg SP Science China Press 01.04.2012
Springer Nature B.V
Subjects
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Diagnostic systems
Fluorescence
Microfluidics
Microprocessors
Real time
Signal to noise ratio
光学检测器
处理器
实时
早期诊断
肺癌
芯片系统
荧光
过滤
lung cancer
confocal detection
microfluidic chip
non-linear filter
isothermal amplification
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Summary:This work presents a promising clinical molecular diagnostics for early stage lung cancer. This novel diagnostic method utilized the loopmediated isothermal amplification （LAMP）, micro fluidic chips and a confocal optical detector with a nonlinear fluorescent filter processor. An isothermal amplification based microfluidic chip for the early diagnostics of lung cancer was developed and a confocal optical detector was improved by a novel real-time fluorescent filter to sensitively monitor the DNA amplification procedure with high signal to noise ratio and fluorescence collecting ability. Experiment showed that a rapid diagnostic of lung cancer by detecting the existence of the CEA mRNA could be performed in only 5 laL of reaction assay in less than 45 min. While the traditional intube RTPCR set consumed more than 25 -μL of the assay and took more than 90 rain.
Bibliography:WANG TongZhou, ZHANG Ye, HUANG GuoLiang, WANG Can, XIE Lan, MA Li, LI ZhiYong, LUO XianBo, TIAN Hao, LI Qiang, LI Xin, LV ZhouYan ＆ BAO XiaoFanI Department of Biomedical Engineering, School of Medicine, Tsinghua University, Beijing 100084, China National Engineering Research Center for Beijing Biochip Technology, Beijing 102206, China Yangtze Delta Region Institute of Tsinghua University, Hangzhou 330401, China Received November 21, 2011; accepted February 2, 2012; published online February 26, 2012
This work presents a promising clinical molecular diagnostics for early stage lung cancer. This novel diagnostic method utilized the loopmediated isothermal amplification （LAMP）, micro fluidic chips and a confocal optical detector with a nonlinear fluorescent filter processor. An isothermal amplification based microfluidic chip for the early diagnostics of lung cancer was developed and a confocal optical detector was improved by a novel real-time fluorescent filter to sensitively monitor the DNA amplification procedure with high signal to noise ratio and fluorescence collecting ability. Experiment showed that a rapid diagnostic of lung cancer by detecting the existence of the CEA mRNA could be performed in only 5 laL of reaction assay in less than 45 min. While the traditional intube RTPCR set consumed more than 25 -μL of the assay and took more than 90 rain.
11-5839/O6
ISSN:1674-7291
1869-1870
DOI:10.1007/s11426-012-4543-8