A New, Nondestructive, Split-Root System for Local and Systemic Plant Responses Studies with Soybean

Plants use long-distance signaling mechanisms to coordinate their growth and control their interactions, positive or negative, with microbes. Split-root systems (SRS) have been used to study the relevance of both local and systemic plant mechanisms that participate in the control of rhizobia-legume...

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Bibliographic Details
Published inMethods in molecular biology (Clifton, N.J.) Vol. 1734; p. 297
Main Authors Hidalgo, Ángeles, Ruiz-Sainz, José E, Vinardell, José M
Format Journal Article
LanguageEnglish
Published United States 2018
Subjects
Germination
Glycine max - physiology
Plant Root Nodulation - physiology
Plant Roots - physiology
Seedlings
Seeds - physiology
Nodulation
Hydroponic culture
Soybean
Autoregulation of nodulation
Split-root system
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Summary:Plants use long-distance signaling mechanisms to coordinate their growth and control their interactions, positive or negative, with microbes. Split-root systems (SRS) have been used to study the relevance of both local and systemic plant mechanisms that participate in the control of rhizobia-legume symbioses. In this work we have developed a modification of the standard split-root system (SRS) used with soybean. This modified method, unlike previous systems, operates in hydroponics conditions and therefore is nondestructive and allows for the continuous monitoring of soybean roots throughout the whole experiment.
ISSN:1940-6029
DOI:10.1007/978-1-4939-7604-1_23