Phenotypic transformation of primary mouse fibroblasts by BPV 1 DNA

Cultures of primary fibroblasts of C57BL/6J mice were used as targets for transformation by bovine papillomavirus type 1 (BPV 1) DNA. Although no foci were observed, several lines of transformed cells were established by subculturing. These immortalized cell lines had in vitro growth characteristics...

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Bibliographic Details
Published inArchives of virology Vol. 100; no. 1-2; p. 17
Main Authors Mäntyjärvi, R, Sarkkinen, H, Parkkinen, S, Ryhänen, A, Karjalainen, H, Syrjänen, K, Syrjänen, S
Format Journal Article
LanguageEnglish
Published Austria 01.01.1988
Subjects
Animals
Bovine papillomavirus 1 - genetics
Cell Line, Transformed - analysis
Cell Line, Transformed - ultrastructure
Cell Transformation, Neoplastic
Cell Transformation, Viral
DNA, Viral - metabolism
Fibroblasts - cytology
Immunologic Techniques
Karyotyping
Mice
Mice, Inbred C57BL
Mice, Nude
Papillomaviridae - genetics
Phenotype
Ploidies
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Summary:Cultures of primary fibroblasts of C57BL/6J mice were used as targets for transformation by bovine papillomavirus type 1 (BPV 1) DNA. Although no foci were observed, several lines of transformed cells were established by subculturing. These immortalized cell lines had in vitro growth characteristics in high and low serum media and saturation densities typical of transformed cells. Karyotype analyses revealed extensive aneuploidic changes. In two of the three cell lines analyzed, viral DNA was present in monomeric episomal form, in the third cell line all viral sequences were found in the high molecular weight region of a Southern blot. Despite the transformed phenotype, only one of the cell lines was tumorigenic in nude mice at a low level.
ISSN:0304-8608
DOI:10.1007/BF01310904