Parallel analysis of individual biological cells using multifocal laser tweezers Raman spectroscopy

We report on the development and characterization of a multifocal laser tweezers Raman spectroscopy (M-LTRS) technique for parallel Raman spectral acquisition of individual biological cells. Using a 785-nm diode laser and a time-sharing laser trapping scheme, multiple laser foci are generated to opt...

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Bibliographic Details
Published inApplied spectroscopy Vol. 64; no. 11; p. 1308
Main Authors Liu, Rui, Taylor, Douglas S, Matthews, Dennis L, Chan, James W
Format Journal Article
LanguageEnglish
Published United States 01.11.2010
Subjects
Cytological Techniques - instrumentation
Equipment Design
Erythrocytes - cytology
Humans
Microspheres
Optical Tweezers
Spectrum Analysis, Raman - methods
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Summary:We report on the development and characterization of a multifocal laser tweezers Raman spectroscopy (M-LTRS) technique for parallel Raman spectral acquisition of individual biological cells. Using a 785-nm diode laser and a time-sharing laser trapping scheme, multiple laser foci are generated to optically trap single polystyrene beads and suspension cells in a linear pattern. Raman signals from the trapped objects are simultaneously projected through the slit of a spectrometer and spatially resolved on a charge-coupled device (CCD) detector with minimal signal crosstalk between neighboring cells. By improving the rate of single-cell analysis, M-LTRS is expected to be a valuable method for studying single-cell dynamics of cell populations and for the development of high-throughput Raman based cytometers.
ISSN:1943-3530
DOI:10.1366/000370210793334972