Determination of platinum in serum and ultrafiltrate by flameless atomic absorption spectrophotometry

A graphite furnace atomic absorption spectrophotometric assay, capable of accurately determining nanogram amounts of platinum in serum and ultrafiltrate, was developed. A sample serum or ultrafiltrate was acidified with nitric acid and heated to destroy the protein-platinum bond. A measured excess o...

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Bibliographic Details
Published inJournal of pharmaceutical sciences Vol. 70; no. 5; p. 500
Main Authors Hull, D A, Muhammad, N, Lanese, J G, Reich, S D, Finkelstein, T T, Fandrich, S
Format Journal Article
LanguageEnglish
Published United States 01.05.1981
Subjects
Humans
Platinum - blood
Spectrophotometry, Atomic - methods
Ultrafiltration
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Summary:A graphite furnace atomic absorption spectrophotometric assay, capable of accurately determining nanogram amounts of platinum in serum and ultrafiltrate, was developed. A sample serum or ultrafiltrate was acidified with nitric acid and heated to destroy the protein-platinum bond. A measured excess of ammonium 1-pyrrolidinedithiocarbamate was added, and the platinum complex was extracted into isopropylacetone. The extract was injected into the graphite furnace. The sample was dried, charred, and atomized using optimal conditions. The resulting absorbance was used to determine the platinum content.
ISSN:0022-3549
1520-6017
DOI:10.1002/jps.2600700509