Isolation of novel bovine parainfluenza virus type 5 (bPIV5) and its incidence in Korean cattle

• Published in: Korean journal of veterinary research Vol. 54; no. 2; pp. 107 - 112
• Main Authors: Dong Kun Yang, Jin Ju Nah, Ha Hyun Kim, Sung Suk Choi, You Chan Bae, Jung Won Park, Jae Young Song
• Format: Journal Article
• Language: English
• Published: 대한수의학회 2014
• Subjects: 수의학; virus isolation; parainfluenza virus 5; bovine; neurologic disease
• ISSN: 2466-1384; 2466-1392
• DOI: 10.14405/kjvr.2014.54.2.107

Four viruses showing cytopathic effects in MDBK cells were isolated from brains of cattle showing downercattle syndrome in 2012. The isolates were confirmed to belong to the genus Rubulavirus of the subfamilyParamyxovirinae. Isolate QIA-B1201 had the ability to hemagglutinate red blood cells from several species of animalsand was capable of adsorbing guinea pig erythrocytes on the surface of infected Vero cells. Nucleotide sequence analysisshowed that two isolates (QIA-B1201 and QIA-B1204) had high similarity with other human and animal PIV5 isolatesranging from 98.1 to 99.8%. The highest sequence similarity of the two isolates corresponded to strain KNU-11 (99.8%at the nucleotide and amino acid level) isolated from suckling piglets in Korea in 2012. To evaluate the virulenceof strain QIA-B1201, we inoculated bPIV5 into 5 week-old mice via both the intraperitoneal and intracranial route. Body weight was not significantly altered in mice inoculated with QIA-B1201. In this study, we isolated andcharacterized novel bPIV5s from brain samples showing downer cattle syndrome, but were not able to elucidate thepathogenicity of the bPIV5s in mice. KCI Citation Count: 2