IMMUNOHISTOCHEMICAL STUDY ON SUBPOPULATIONS OF B CELLS IN THE NASAL MUCOSA

• Published in: Nippon Jibi Inkoka Gakkai Kaiho Vol. 99; no. 5; pp. 695 - 699,723
• Main Author: TAKASAKI, KENJI
• Format: Journal Article
• Language: English; Japanese
• Published: Japan The Oto-Rhino-Laryngological Society of Japan, Inc 01.05.1996
• Subjects: Adolescent; Adult; Antigens, CD20 - metabolism; B cell; B-Lymphocyte Subsets - immunology; CD20; CD40; CD40 Antigens - metabolism; Child; Female; Humans; immunohistochemical study; Immunohistochemistry; Male; nasal mucosa; Nasal Mucosa - immunology
• ISSN: 0030-6622; 1883-0854
• DOI: 10.3950/jibiinkoka.99.695

The subpopulations of B-lymphocytes in the inferior turbinates of 12 patients with allergic rhinitis and 3 patients with nonallergic nasal disease were studied by immunohistochemical procedures. The specimens were frozen at-70°C and sliced at 4μm with a cryostat. The LSAB method with monoclonal antibodies (CD4, CD8, CD20, CD40) was used to detect subpopulations of T-lymphocytes and B-lymphocytes. In every subject the number of CD4 (+) cells dominated over that of CD8 (+) cells in the lamina propria. CD20 (+) cells were seen mainly in the border between the subepithelial layer and the nasal gland layer. CD40 (+) cells were found among the epithelial cells, the gland cells and the round mononuclear cells. In the serial sections CD20 (+) CD40 (+) cells were found in the inferior turbinates. We did not observe any difference in the distribution of CD20 (+) cells and expression of CD40 between allergic patients and nonallergic patients. Further study seems to be necessary to elucidate the relationship between CD20 (+) CD40 (+) cells and T-lymphocytes and some cytokines.