Response of Escherichia coli Containing Mycobacterial Carotene Genes to UV Radiation

The plasmid pC5, which encodes biogenesis of lycopene in Mycobacterium aurum A(+), was partially digested by restriction endonucleases and generated fragments were cloned. After transformation of Escherichia coli (colorless bacteria) with the plasmids so constructed, seven orange clones were detecte...

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Published inBioMed research international Vol. 1; no. 2; pp. 79 - 84
Main Authors Houssaini-Iraqui, Mohamed, Khamlichi, Naima, El Yamani, Jamal, Rastogi, Nalin
Format Journal Article
LanguageEnglish
Published United States Hindawi Publishing Corporation 2001
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Summary:The plasmid pC5, which encodes biogenesis of lycopene in Mycobacterium aurum A(+), was partially digested by restriction endonucleases and generated fragments were cloned. After transformation of Escherichia coli (colorless bacteria) with the plasmids so constructed, seven orange clones were detected and found to carry the same recombinant plasmid (pC51). E. coli cells containing this plasmid synthesize neurosporene and lycopene, and were more resistant to ultraviolet irradiation than non pigmented strain.
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ISSN:2314-6133
1110-7243
1110-7251
2314-6141
DOI:10.1155/S1110724301000225