Electrochemical/photochemical formation of enzyme patterns on glass substrates using a scanning electrochemical/confocal microscope

A hybrid system consisting of a scanning electrochemical microscope (SECM) and scanning confocal microscope (SCFM) was fabricated and used for micropatterning and imaging of diaphorase immobilized on a glass substrate. Simultaneous imaging of the diaphorase spots demonstrated that the SECM can provi...

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Published inSensors and actuators. B, Chemical Vol. 91; no. 1; pp. 199 - 204
Main Authors Oyamatsu, Daisuke, Kanaya, Norihiro, Shiku, Hiroshi, Nishizawa, Matsuhiko, Matsue, Tomokazu
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.06.2003
Subjects
Biosensors
Confocal microscopy
Dehydrolipoamide dehydrogenase
Diaphorase
Glass
Immobilization
Patterning
Q1
SCFM
SECM
SCFM
Patterning
SECM
Diaphorase
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Summary:A hybrid system consisting of a scanning electrochemical microscope (SECM) and scanning confocal microscope (SCFM) was fabricated and used for micropatterning and imaging of diaphorase immobilized on a glass substrate. Simultaneous imaging of the diaphorase spots demonstrated that the SECM can provide information on a localized enzyme reaction, while the SCFM affords information on the location of the active enzyme. By using this SECM/SCFM system, spatially selective deactivation of diaphorase was performed by inducing a local electrochemical reaction or by irradiating with the focused laser. The resulting patterns of diaphorase were simultaneously imaged with the SECM/SCFM system.
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ISSN:0925-4005
1873-3077
DOI:10.1016/S0925-4005(03)00089-3