Isozyme polymorphism and virulence of Indian isolates of the rice sheath blight fungus

• Published in: Mycopathologia (1975) Vol. 156; no. 2; pp. 101 - 108
• Main Authors: NEERAJA, C. N, SHENOY, V. V, REDDY, C. S, SARMA, N. P
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.01.2003; Springer Nature B.V
• Subjects: Biological and medical sciences; Cluster Analysis; Cultivars; Disease; Fundamental and applied biological sciences. Psychology; Fungi; India; Isoenzymes - genetics; Microbiology; Oryza; Pathogens; Phylogeny; Plant Diseases - microbiology; Plant Proteins - genetics; Plant Proteins - metabolism; Polymorphism; Polymorphism, Genetic; Rhizoctonia - enzymology; Rhizoctonia - genetics; Rhizoctonia - pathogenicity; Variance analysis; Virulence; India; sheath blight; Rhizoctonia; Isozyme polymorphism; Microbiology; virulence; rice
• ISSN: 0301-486X; 1573-0832
• DOI: 10.1023/A:1022988710654

Inadequate information about the genetic structure of the polyphagous Rhizoctonia solani has made sheath blight resistance breeding a difficult task. To assess the variability in the Indian populations of sheath blight fungus, 18 isolates were collected from different rice growing regions of India and analyzed for virulence and electrophoretic profiles of 13 isozymes. The virulence spectrum of all 18 isolates was examined on susceptible IR50 and tolerant Swarnadhan varieties, based on which the isolates could be grouped as highly virulent, moderately virulent or avirulent. A total of 11 enzyme systems with 153 electrophoretic phenotypes were applied to characterize the genetic variation among the isolates. Cluster analyses based on isozyme patterns resulted in one major cluster comprising 16 virulent isolates, with two avirulent isolates loosely linked to this at 0.13 similarity. Isozyme systems of esterases (both alpha and beta) and 6-phosphogluconic dehydrogenase could be used to fingerprint the individual isolates.