Biomineralization of Cu2S Nanoparticles by Geobacter sulfurreducens

• Published in: Applied and environmental microbiology Vol. 86; no. 18
• Main Authors: Kimber, Richard L, Bagshaw, Heath, Smith, Kurt, Buchanan, Dawn M, Coker, Victoria S, Cavet, Jennifer S, Lloyd, Jonathan R
• Format: Journal Article
• Language: English
• Published: Washington American Society for Microbiology 01.09.2020
• Subjects: Absorption; Anoxic conditions; BASIC BIOLOGICAL SCIENCES; Biogeochemistry; bioreduction; Cadmium; Catalysts; Cell surface; Cell suspensions; Contaminants; Copper; Copper sulfides; Cu2S; Energy dispersive X ray spectroscopy; Fine structure; Geobacter sulfurreducens; Geomicrobiology; Image processing; Lead; Mercury; Mercury (metal); Metals; Microorganisms; Mineralization; Nanoparticles; Pollution control; Sediment pollution; Soil contamination; Soil dynamics; Soils; Sulfate reduction; Sulfates; Sulfur; Transmission electron microscopy; Ultrastructure; X ray absorption; X-ray spectroscopy
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.00967-20

Biomineralization of Cu has been shown to control contaminant dynamics and transport in soils. However, very little is known about the role that subsurface microorganisms may play in the biogeochemical cycling of Cu. In this study, we investigate the bioreduction of Cu(II) by the subsurface metal-reducing bacterium Geobacter sulfurreducens. Rapid removal of Cu from solution was observed in cell suspensions of G. sulfurreducens when Cu(II) was supplied, while transmission electron microscopy (TEM) analyses showed the formation of electron-dense nanoparticles associated with the cell surface. Energy-dispersive X-ray spectroscopy (EDX) point analysis and EDX spectrum image maps revealed that the nanoparticles are rich in both Cu and S. This finding was confirmed by X-ray absorption near-edge structure (XANES) and extended X-ray absorption fine structure (EXAFS) analyses, which identified the nanoparticles as Cu2S. Biomineralization of CuxS nanoparticles in soils has been reported to enhance the colloidal transport of a number of contaminants, including Pb, Cd, and Hg. However, formation of these CuxS nanoparticles has only been observed under sulfate-reducing conditions and could not be repeated using isolates of implicated organisms. As G. sulfurreducens is unable to respire sulfate, and no reducible sulfur was supplied to the cells, these data suggest a novel mechanism for the biomineralization of Cu2S under anoxic conditions. The implications of these findings for the biogeochemical cycling of Cu and other metals as well as the green production of Cu catalysts are discussed.