Polyamine sparing may be involved in the prolongation of cell division due to inhibition of phenylpropanoid synthesis in cytokinin-starved soybean cells

• Published in: Journal of plant growth regulation Vol. 16; no. 2; pp. 89 - 93
• Main Authors: MADER, J. C, HANKE, D. E
• Format: Journal Article
• Language: English
• Published: New York, NY Springer 01.05.1997; Springer Nature B.V
• Subjects: acide propionique; acido propionico; amonio fenilalanina liasa; analisis cuantitativo; analyse quantitative; Biological and medical sciences; biosintesis; biosynthese; biosynthesis; carence en substance nutritive; cell culture; Cell division; cells; cellule; celulas; Chemical agents; citoquininas; compose phenolique; compuestos fenolicos; crecimiento; croissance; cultivo de celulas; culture de cellule; cytokinine; cytokinins; deficiencias nutritivas; division cellulaire; division celular; enzyme inhibitors; espermidina; espermina; Fundamental and applied biological sciences. Psychology; Glycine max; growth; inhibidores de enzimas; inhibiteur d' enzyme; Liquid chromatography; nutrient deficiencies; phenolic compounds; Phenols; phenylalanine ammonia lyase; Plant growth; Plant growth substances; Plant physiology and development; Polyamines; propionic acid; putrescina; putrescine; quantitative analysis; Soybeans; spermidine; spermine; Vegetative apparatus, growth and morphogenesis. Senescence; Cell culture; Phenylalanine ammonia-lyase; Polyamine; Enzyme; Growth; Cell division; Enzyme inhibitor; Lyases; Glycine max; Carbon-nitrogen lyases; Ammonia-lyases; Grain legume; Regulation(control); Leguminosae; Dicotyledones; Angiospermae; Spermatophyta; Biological accumulation; Organic hydroxylamine
• ISSN: 0721-7595; 1435-8107
• DOI: 10.1007/PL00006983

Effects on growth, mostly of an inhibitory nature, have been attributed to phenolic compounds in vivo and in vitro. This suggests that L-alpha-aminooxy-beta-phenylpropionic acid (L-AOPP), a competitive inhibitor of phenylalanine ammonia-lyase (PAL), the enzyme controlling the first step in phenylpropanoid synthesis, might stimulate growth in soybean suspension cultures (Glycine max, cv. Acme). The promotive effect of L-AOPP, measured as an increase in cell number, was more clearly detected in the growth-limiting condition of cytokinin starvation. At least one more cell division cycle was completed in the presence of L-AOPP before growth by division ceased and growth continued by expansion only. Phenolic acids are known to conjugate with polyamines, modulating the free levels of these plant growth substances. Thus, the effect of L-AOPP on the titers of free and conjugated polyamines (putrescine, spermidine, and spermine) was investigated by high performance liquid chromatography in the course of cytokinin starvation. An increased level of free putrescine was detected in the presence of L-AOPP relative to controls, especially in the initial period before growth became restricted to cell expansion. The decrease in free putrescine associated with the cessation of cell division was temporarily delayed, suggesting that an interaction between phenolic acids and polyamines is involved in the mechanism of growth promotion by L-AOPP.