Determination of Lead in Urine by Atomic Absorption Spectrophotometry
A method for the determination of lead in urine by means of atomic absorption spectrophotometry (AAS) is described. A combination of wet ashing and extraction with ammonium pyrrolidine dithiocarbamate into isobutylmethylketone was used. The sensitivity was about 0·02 μg./ml. for 1% absorption, and t...
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Published in | British Journal of Industrial Medicine Vol. 25; no. 2; pp. 139 - 143 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
England
BMJ Publishing Group Ltd
01.04.1968
British Medical Association |
Subjects | |
Online Access | Get full text |
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Summary: | A method for the determination of lead in urine by means of atomic absorption spectrophotometry (AAS) is described. A combination of wet ashing and extraction with ammonium pyrrolidine dithiocarbamate into isobutylmethylketone was used. The sensitivity was about 0·02 μg./ml. for 1% absorption, and the detection limit was about 0·02 μg./ml. with an instrumental setting convenient for routine analyses of urines. Using the scale expansion technique, the detection limit was below 0·01 μg./ml., but it was found easier to determine urinary lead concentrations below 0·05 μg./ml. by concentrating the lead in the organic solvent by increasing the volume of urine or decreasing that of the solvent. The method was applied to fresh urines, stored urines, and to urines, obtained during treatment with chelating agents, of patients with lead poisoning. Urines with added inorganic lead were not used. The results agreed well with those obtained with a colorimetric dithizone extraction method (r = 0·989). The AAS method is somewhat more simple and allows the determination of smaller lead concentrations. |
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Bibliography: | href:oemed-25-139.pdf istex:205E651F19C38E3E56C69A9F1B5473BDB846E1BB ark:/67375/NVC-GVMV2XJ4-1 Aided by grants No. B-66-374 and No. B-67-61P-2094-01 from the Swedish Medical Research Council and by a grant from Forsakringsforetaget Folksam, Stockholm. PMID:5647975 local:oemed;25/2/139 |
ISSN: | 0007-1072 1351-0711 1470-7926 |
DOI: | 10.1136/oem.25.2.139 |