Comparison between modified DNA extraction protocols and commercial isolation kits in grapevine (Vitis vinifera L.)

Various protocols have been developed and used for DNA extraction in grapevine. However, owing to the long duration of the isolation steps in previously developed protocols, researchers have preferred to use isolation kits for studies in recent years. In our study, the DNA yield and purity obtained...

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Bibliographic Details
Published inGenetics and molecular research Vol. 11; no. 3; pp. 2343 - 2351
Main Author Akkurt, M
Format Journal Article
LanguageEnglish
Published Brazil 01.01.2012
Subjects
DNA, Plant - isolation & purification
Microsatellite Repeats - genetics
Molecular Biology - methods
Polymerase Chain Reaction
Reagent Kits, Diagnostic
Spectrophotometry
Vitis - genetics
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Summary:Various protocols have been developed and used for DNA extraction in grapevine. However, owing to the long duration of the isolation steps in previously developed protocols, researchers have preferred to use isolation kits for studies in recent years. In our study, the DNA yield and purity obtained using six methods--namely three DNA isolation protocols and three commercial DNA isolation kits--were compared. Modifications were made and the isolation steps were shortened in the previously developed DNA isolation protocols to achieve more rapid and practical protocols. The samples were taken from plants grown under vineyard and greenhouse conditions in two periods during spring and autumn. The best results among the six DNA isolation methods were discussed. The results were also supported with polymerase chain reaction analyses conducted with isolated DNAs.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:1676-5680
1676-5680
DOI:10.4238/2012.August.13.8